microbiomee

  16S rRNA Sequence Carl Woese and George E Fox was the first to used the 16S rRNA gene in bacterial phylogenetic analysis in 1977. This method revolutionized the field of microbiology in identification of unknown microorganisms.  16S ribosome RNA is a subunit of a 30S ribosome RNA, It plays a remarkable role in bacterial translation. It plays a major role in the translation part. This ribosome acts with the 23S subunit and helps in the binding of 50S and 30S ribosomes. Which in turn initiates the protein synthesis. The 16S rRNA has a unique kind of sequence known as the anti shine-dalgarno sequence which binds to the AUG of the mRNA to initiate protein synthesis. The 16S rRNA gene has two parts one is a nonvariable domains and is common in all bacteria. But the other domain is a highly variable region and this is why this part is used in the classification of bacterial species. At present there are many primers available for performing PCR study in 16S rRNA sequencing a...

Microbiology is the study of living organisms of microscopic size. Which include bacteria, fungi, algae, protozoa and infectious agents at the borderline of life that are called viruses. Microorganisms are closely associated with the health and welfare of human beings, some microorganisms are beneficial and others are detrimental. For example, microorganisms are involved in the making of yogurt, cheese and wine. Microorganism can cause disease, spoil food and deteriorate materials like iron pipes, glass lenses and wood pilings. Antony Van  Leeuwenhoek’s report on the ubiquity of microbes enabled Louis Pasteur to discover the involvement of these creatures in fermentation reaction and allowed Robert Koch, Theobald smith,  and many others to discover the association of microbes with disease. Koch is remembered for his isolation of the bacteria that cause anthrax and tuberculosis and for the rigid criteria he demanded before a s...